PUBLICATION

The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary

Authors
Gutnick, A., Blechman, J., Kaslin, J., Herwig, L., Belting, H.G., Affolter, M., Bonkowsky, J.L., and Levkowitz, G.
ID
ZDB-PUB-111027-47
Date
2011
Source
Developmental Cell   21(4): 642-654 (Journal)
Registered Authors
Affolter, Markus, Belting, Heinz-Georg Paul (Henry), Blechman, Janna, Bonkowsky, Joshua, Gutnick, Amos, Kaslin, Jan, Levkowitz, Gil
Keywords
none
MeSH Terms
  • Endothelium, Vascular/cytology
  • Endothelium, Vascular/drug effects*
  • Endothelium, Vascular/metabolism
  • Hypothalamus/drug effects*
  • Hypothalamus/growth & development
  • Hypothalamus/metabolism
  • Cells, Cultured
  • Immunoenzyme Techniques
  • Pituitary Gland/blood supply*
  • Pituitary Gland/cytology*
  • Pituitary Gland/drug effects
  • Oxytocin/pharmacology*
  • Animals
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Hypothalamo-Hypophyseal System/cytology
  • Hypothalamo-Hypophyseal System/drug effects
  • Hypothalamo-Hypophyseal System/metabolism
  • Gene Expression Regulation, Developmental/drug effects
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Neurons/cytology
  • Neurons/drug effects*
  • Neurons/metabolism
  • Animals, Genetically Modified
  • Zebrafish/embryology
  • Oxytocics/pharmacology
(all 28)
PubMed
22014522 Full text @ Dev. Cell
Abstract
The hypothalamo-neurohypophyseal system (HNS) is the neurovascular structure through which the hypothalamic neuropeptides oxytocin and arginine-vasopressin exit the brain into the bloodstream, where they go on to affect peripheral physiology. Here, we investigate the molecular cues that regulate the neurovascular contact between hypothalamic axons and neurohypophyseal capillaries of the zebrafish. We developed a transgenic system in which both hypothalamic axons and neurohypophyseal vasculature can be analyzed in vivo. We identified the cellular organization of the zebrafish HNS as well as the dynamic processes that contribute to formation of the HNS neurovascular interface. We show that formation of this interface is regulated during development by local release of oxytocin, which affects endothelial morphogenesis. This cell communication process is essential for the establishment of a tight axovasal interface between the neurons and blood vessels of the HNS. We present a unique example of axons affecting endothelial morphogenesis through secretion of a neuropeptide.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39standard conditionsProtruding-mouth
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39standard conditionsProtruding-mouth
c264Tg; s843Tg; zc57Tgstandard conditionsProtruding-mouth
c264Tg; s843Tg; zc57Tgstandard conditionsProtruding-mouth
c264Tg; s843Tg; zc57Tgstandard conditionsProtruding-mouth
c264Tg; s843Tg; zc57Tgchemical treatment: metronidazoleProtruding-mouth
c264Tg; s843Tg; zc57Tgchemical treatment: metronidazoleProtruding-mouth
c264Tg; s843Tg; zc57Tgchemical treatment: metronidazoleProtruding-mouth
c264Tg; s843Tg; zc57Tgchemical treatment: metronidazoleProtruding-mouth
s843Tg; zc57Tgchemical treatment: metronidazoleProtruding-mouth
1 - 10 of 19
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
c264TgTransgenic Insertion
    m39
      Deficiency
      rk8TgTransgenic Insertion
        s843TgTransgenic Insertion
          s896TgTransgenic Insertion
            sd2TgTransgenic Insertion
              ubs4TgTransgenic Insertion
                um13TgTransgenic Insertion
                  wz01TgTransgenic Insertion
                    y7TgTransgenic Insertion
                      1 - 10 of 14
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      oxtMO2-oxtMRPHLNO
                      oxtraMO1-oxtraMRPHLNO
                      1 - 2 of 2
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                      Fish
                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab2-oxtpolyclonalGuinea pig
                      1 - 1 of 1
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                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      DsRedEFGDsRed
                      DsRedxEFGDsRedx
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GAL4FFEFGGAL4FF
                      GFPEFGGFP
                      KaedeEFGKaede
                      mCherryEFGmCherry
                      NTREFGNTR
                      RFPEFGRFP
                      1 - 10 of 10
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                      Mapping
                      No data available