FIGURE

Fig. 2

ID

ZDB-FIG-111101-36

Publication

Deng et al., 2011 - Dual roles for Rac2 in neutrophil motility and active retention in zebrafish hematopoietic tissue

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Fig. 2

Rac2D57N-Expressing Zebrafish Larvae Are More Susceptible to Bacterial Infection

(A) Left, demonstration of zebrafish larvae otic infection. Phenol red tracking dye (pink) was used to visualize bacterial infection. Sudan black staining of neutrophil recruitment 2 hr post ear infection (hpi) in Rac2 (middle) and D57N larvae (right) larvae. Scale bar represents 50 μm.

(B) Neutrophils recruited to the ear 2 hpi were quantified. n = 41 (Rac2) and 39 (D57N). p < 0.001, two-tailed Mann-Whitney U test.

(C) Neutrophils recruited to the ear 1 hpi were quantified in control and Rac2 morphants. n = 36 (Ctrl MO) and 57 (Rac2 MO). p < 0.001, two-tailed Mann-Whitney U test.

(D) Survival of larvae challenged with <5 × 10⁴ cfu of PAK (pMKB1::mCherry). n = 16 (Rac2) and 18 (D57N).

(E) Recruitment of leukocytes to otic infection in Rac2 and D57N larvae. Zebrafish larvae from Tg(mpx:dendra2) crossed with Rac2 or D57N lines were infected with PAK (<10⁴ cfu) into the left ear. Total leukocyte populations were immunostained with L-Plastin antibody. Neutrophils were visualized by Dendra2 green fluorescence. The right ear of the same fish was shown as control. Images are representative for at least 15 individual fish. Scale bar represents 50 μm.

Expression Data

Genes:	Dendra2 lcp1
Fish:	uwm4Tg; zf306Tg uwm4Tg; zf307Tg
Condition:	bacterial treatment by injection: Pseudomonas aeruginosa
Anatomical Terms:	leukocyte neutrophil
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-rac2 zf307Tg (AB) uwm4Tg; zf307Tg
Condition:	bacterial treatment by injection: Pseudomonas aeruginosa
Knockdown Reagent:	MO1-rac2
Observed In:	defense response to Gram-negative bacterium neutrophil neutrophil chemotaxis neutrophil mediated immunity whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 21(4), Deng, Q., Yoo, S.K., Cavnar, P.J., Green, J.M., and Huttenlocher, A., Dual roles for Rac2 in neutrophil motility and active retention in zebrafish hematopoietic tissue, 735-745, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell