Fig. S2

Analysis of reporter gene expression in other tissues than the pectoral fins: the zfprrx1aCNE:EGFP lines. A–E: zfprrx1a(cFos):EGFP line#2. Apart from the expression in the developing pectoral fins from 27 hours postfertilization (hpf) onward (marked by arrowheads in C–E), enhanced green fluorescent protein (EGFP) signal is observed in (i) part of the somites of the 18 hpf embryo (so, in A) and in (ii) the otic vesicle and anterior lateral line from 24 hpf onward (arrows in B–E and Fig. 5 A–C). F–J: zfprrx1a(cFos):EGFP line#6. Apart from the expression in the developing pectoral fins from 27 hpf onward (marked here by arrowheads in H–J), EGFP signal is also observed in the anterior brain and retina from 24 hpf onward (arrows in G–J). The specific sites of reporter gene expression in each of the transgenic lines may represent positional effects due to different integration sites of the transgene. Reporter gene expression is also detected in common areas in the two transgenic lines, which are some mesenchymal cells in the median fin fold and scattered cells surrounding the somatic tissue (mff and double arrowheads(Logan et al., 2002). Nonspecific expression in the dorsal-most aspect of the yolk extension is observed in these transgenic lines (A–J).