PUBLICATION

The Prx1 limb enhancers: Targeted gene expression in developing zebrafish pectoral fins

Authors
Hernández-Vega, A., and Minguillón, C.
ID
ZDB-PUB-110628-33
Date
2011
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   240(8): 1977-88 (Journal)
Registered Authors
Minguillón, Carolina
Keywords
teleost, zebrafish, transgenesis, prx1, prrx1, pectoral fin development, limb development
MeSH Terms
  • Animals
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Embryo, Nonmammalian/anatomy & histology
  • Embryo, Nonmammalian/physiology
  • Homeodomain Proteins/genetics*
  • Morphogenesis*
  • Zebrafish*/anatomy & histology
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Gene Expression Regulation, Developmental*
  • Sequence Alignment
  • Genes, Reporter
  • Base Sequence
  • Molecular Sequence Data
  • Animals, Genetically Modified
  • Promoter Regions, Genetic
  • Extremities/anatomy & histology*
  • Extremities/embryology*
  • Mice
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
(all 22)
PubMed
21674688 Full text @ Dev. Dyn.
Abstract
Limbs represent an excellent model to study the induction, growth, and patterning of several organs. A breakthrough to study gene function in various tissues has been the characterization of regulatory elements that allow tissue-specific interference of gene function. The mouse Prx1 promoter has been used to generate limb-specific mutants and overexpress genes in tetrapod limbs. Although zebrafish possess advantages that favor their use to study limb morphogenesis, there is no driver described suitable for specifically interfering with gene function in developing fins. We report the generation of zebrafish lines that express enhanced green fluorescent protein (EGFP) driven by the mouse Prx1 enhancer in developing pectoral fins. We also describe the expression pattern of the zebrafish prrx1 genes and identify three conserved non-coding elements (CNEs) that we use to generate fin-specific EGFP reporter lines. Finally, we show that the mouse and zebrafish regulatory elements may be used to modify gene function in pectoral fins.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
No data available
Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
Marker Marker Type Name
EGFPEFGEGFP
1 - 1 of 1
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Mapping
No data available