PUBLICATION

The Prx1 limb enhancers: Targeted gene expression in developing zebrafish pectoral fins

Authors

Hernández-Vega, A., and Minguillón, C.

ID

ZDB-PUB-110628-33

Date

2011

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 240(8): 1977-88 (Journal)

Registered Authors

Minguillón, Carolina

Keywords

teleost, zebrafish, transgenesis, prx1, prrx1, pectoral fin development, limb development

MeSH Terms

Animals
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Embryo, Nonmammalian/anatomy & histology
Embryo, Nonmammalian/physiology
Homeodomain Proteins/genetics*
Morphogenesis*
Zebrafish*/anatomy & histology
Zebrafish*/embryology
Zebrafish*/genetics
Gene Expression Regulation, Developmental*
Sequence Alignment
Genes, Reporter
Base Sequence
Molecular Sequence Data
Animals, Genetically Modified
Promoter Regions, Genetic
Extremities/anatomy & histology*
Extremities/embryology*
Mice
Recombinant Fusion Proteins/genetics
Recombinant Fusion Proteins/metabolism

(all 22)

PubMed

21674688 Full text @ Dev. Dyn.

Abstract

Limbs represent an excellent model to study the induction, growth, and patterning of several organs. A breakthrough to study gene function in various tissues has been the characterization of regulatory elements that allow tissue-specific interference of gene function. The mouse Prx1 promoter has been used to generate limb-specific mutants and overexpress genes in tetrapod limbs. Although zebrafish possess advantages that favor their use to study limb morphogenesis, there is no driver described suitable for specifically interfering with gene function in developing fins. We report the generation of zebrafish lines that express enhanced green fluorescent protein (EGFP) driven by the mouse Prx1 enhancer in developing pectoral fins. We also describe the expression pattern of the zebrafish prrx1 genes and identify three conserved non-coding elements (CNEs) that we use to generate fin-specific EGFP reporter lines. Finally, we show that the mouse and zebrafish regulatory elements may be used to modify gene function in pectoral fins.

Genes / Markers

Figures