header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-110622-77
Fish et al., 2011 - A Slit/miR-218/Robo regulatory loop is required during heart tube formation in zebrafish. Development (Cambridge, England)   138(7):1409-1419 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 20-25 somites

Fig. 4

Heart field migration is sensitive to Robo1 dosage. (A) Endocardial migration was defective at 20 somites in zebrafish embryos injected with 75 pg Robo1 mRNA (mCherry mRNA was utilized as a control). (B) Rescue of migration defects in miR-218 morphants was accomplished by co-injecting sub-phenotypic doses of robo1 MO. Quantification of migration defects is shown beneath. *, P<0.05 (Fisher′s exact test for a 2×3 contingency table). (C) Analysis of endocardial migration at the 20-somite stage in Tg(hsp70l:slit2-GFP) embryos that were heat shocked at 5 somites. Embryos lacking the transgene were used as a control. Disorganization of the endocardium was evident in Slit2-overexpressing embryos. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP s896Tg; twu26Tg control 20-25 somites myocardium IFL
s896Tg; twu26Tg + MO1-mir218 standard conditions 20-25 somites myocardium IFL
s896Tg; twu26Tg + MO1-mir218 + MO1-robo1 standard conditions 20-25 somites myocardium IFL
mCherry rw015aTg; s896Tg control 20-25 somites endocardium IFL
s896Tg control 20-25 somites endocardium IFL
s896Tg; twu26Tg control 20-25 somites endocardium IFL
s896Tg; twu26Tg + MO1-mir218 standard conditions 20-25 somites endocardium IFL
s896Tg; twu26Tg + MO1-mir218 + MO1-robo1 standard conditions 20-25 somites endocardium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rw015aTg; s896Tg control 20-25 somites cardioblast migration decreased process quality, abnormal
rw015aTg; s896Tg control 20-25 somites endocardium disorganized, abnormal
rw015aTg; s896Tg control 20-25 somites endocardium formation decreased process quality, abnormal
s896Tg; twu26Tg + MO1-mir218 standard conditions 20-25 somites cardioblast migration decreased process quality, abnormal
20-25 somites endocardium mislocalised, abnormal
s896Tg; twu26Tg + MO1-mir218 + MO1-robo1 standard conditions 20-25 somites cardioblast migration delayed, abnormal
20-25 somites endocardium position, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development