Fig. 7

Early and late expression patterns of the adenohypophyseal marker lim3 in MO prop1 (MO; B/D/H) and control (Co; A/C/G) injected embryos, and rescue experiments at 24 hpf (E/F). At early stages (20 and 24 hpf), a drastic reduction of lim3 expression was observed in the pituitary anlage but not in the spinal cord motoneurons of prop1 knock-down embryos compared to controls (B/D vs. A/C). Lim3 expression in the adenohypophysis was partially rescued by co-injecting prop1 MO with 100 pg of cRNA (E, arrows); in vivo integrity of prop1 cRNA was confirmed by ISH using a prop1 mRNA probe (F). At 48 hpf, adenohypophyseal lim3 expression of morphants was indistinguishable from that of controls (H vs. G). Gene names are indicated in the lower left-hand corner of each panel. Age in hpf and (treatments) in the upper right-hand corner of each panel. Pituitary (pit); spinal cord (Spc). Scale bar for (A/B; E/F; G/H) = 100 μm; (C/D) = 200 μm.