FIGURE

Fig. 3

ID

ZDB-FIG-110527-30

Publication

Hong et al., 2011 - The transcriptional mediator component med12 is required for hindbrain boundary formation

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Fig. 3

Specific loss of hindbrain boundaries in kto^y82 mutants.

Lateral (A–D,G,H) and dorsal views (E,F,I–L) of wt (A,C,E,G,I,K) and kto^y82 mutant embryos (B,D,F,H,J,L) at 18 hpf (A,B) and 24 hpf (C–L). (A–F, K,L) in situ hybridization. (A,B) Expression of the earliest hindbrain boundary marker foxb1.2. (C–F) Completely loss of rfng expression in hindbrain boundaries. (G–J) Confocal images of hindbrain boundary neurons; immunostaining with zn5 (red) in pGFP-5.3 transgenic zebrafish (green) (G,H); staining with zebrafish delta D antibody, zdD2 (I,J). (K,L) notch1a expression in the hindbrain. mhb, mid-hindbrain boundary; ov, otic vesicle; r, rhombomere.

Expression Data

Genes:	foxb1a GFP notch1a rfng
Antibodies:	Ab1-dld zn-5
Fish:	WT med12^y82/y82 e1Tg med12^y82/y82; e1Tg
Anatomical Terms:	hindbrain hindbrain neuron midbrain hindbrain boundary rhombomere compartment boundary rhombomere 3 rhombomere 5
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	med12^y82/y82 med12^y82/y82; e1Tg
Observed In:	hindbrain commissure neuron rhombomere boundary formation
Stage Range:	14-19 somites to Prim-5

Phenotype Detail

Acknowledgments

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