ZFIN ID: ZDB-FIG-110527-30
Hong et al., 2011 - The transcriptional mediator component med12 is required for hindbrain boundary formation. PLoS One   6(4):e19076 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 3 Specific loss of hindbrain boundaries in ktoy82 mutants.

Lateral (A–D,G,H) and dorsal views (E,F,I–L) of wt (A,C,E,G,I,K) and ktoy82 mutant embryos (B,D,F,H,J,L) at 18 hpf (A,B) and 24 hpf (C–L). (A–F, K,L) in situ hybridization. (A,B) Expression of the earliest hindbrain boundary marker foxb1.2. (C–F) Completely loss of rfng expression in hindbrain boundaries. (G–J) Confocal images of hindbrain boundary neurons; immunostaining with zn5 (red) in pGFP-5.3 transgenic zebrafish (green) (G,H); staining with zebrafish delta D antibody, zdD2 (I,J). (K,L) notch1a expression in the hindbrain. mhb, mid-hindbrain boundary; ov, otic vesicle; r, rhombomere.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxb1a WT standard conditions 14-19 somites rhombomere compartment boundary ISH
med12y82/y82 standard conditions 14-19 somites rhombomere compartment boundary ISH
GFP e1Tg standard conditions Prim-5 midbrain hindbrain boundary IFL
Prim-5 rhombomere 3 IFL
Prim-5 rhombomere 5 IFL
med12y82/y82; e1Tg standard conditions Prim-5 midbrain hindbrain boundary IFL
Prim-5 rhombomere 3 IFL
Prim-5 rhombomere 5 IFL
notch1a WT standard conditions Prim-5 hindbrain ISH
med12y82/y82 standard conditions Prim-5 Not Detected hindbrain ISH
rfng WT standard conditions Prim-5 rhombomere compartment boundary ISH
med12y82/y82 standard conditions Prim-5 Not Detected rhombomere compartment boundary ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-dld IHC WT standard conditions Prim-5 hindbrain
IHC med12y82/y82 standard conditions Prim-5 Not Detected hindbrain
zn-5 IHC e1Tg standard conditions Prim-5 hindbrain neuron
IHC med12y82/y82; e1Tg standard conditions Prim-5 hindbrain neuron
Phenotype Details
Fish Conditions Stage Phenotype
med12y82/y82 standard conditions 14-19 somites rhombomere boundary formation disrupted, abnormal
Prim-5 rhombomere boundary formation disrupted, abnormal
med12y82/y82; e1Tg standard conditions Prim-5 hindbrain commissure neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One