Fig. 3

The transplanted donor PGC localized at the gonadal region of the host embryo and started to proliferate at the same time as the host PGCs during gonadal development. Donor and host PGCs were labeled with GFP or RFP, respectively. The lower column showed an enlarged image of the boxed areas in the upper column. (A) 24 hpf chimera. A transplanted single PGC was localized, along with host PGCs, at the position where the gonad will develop. (B) 7 dpf chimera. A GFP-labeled PGC had divided once at this stage (arrow) and some of the host PGCs had also started to proliferate. PGCs still lay loosely at this stage. (C) 11 dpf chimera. Both donor and host PGCs continued to proliferate and the PGCs began to form a tighter cluster than previously. (D) 14 dpf chimera. Both donor and host PGCs proliferated and became smaller. Arrows indicate a transplanted single donor PGC. Arrowheads indicate reflected fluorescence of labeled PGCs on gas bladder. Scale bar in upper column indicates 500 μm. Scale bar in lower column indicates 100 μm.