FIGURE

Fig. S4

ID

ZDB-FIG-110221-36

Publication

Harrison et al., 2011 - The epigenetic regulator Histone Deacetylase 1 promotes transcription of a core neurogenic programme in zebrafish embryos

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Fig. S4

Efficient immunoprecipitation of Hdac1 protein from embryonic chromatin by anti-Hdac1 antibody. Crosslinked, sonicated chromatin was prepared from 12 hpf zebrafish embryos, then incubated with anti-Hdac1 antibody and negative control IgG. Immune complexes were precipitated with Protein G-agarose and both immunoprecipitated proteins and unbound proteins were analysed by SDS-PAGE and Western blotting with the anti-Hdac1 antibody, with an equivalent sample of input chromatin run alongside. All Hdac1 protein in the input sample was recovered in the anti-Hdac1 immunoprecipitate, whereas none of the Hdac1 protein was immunoprecipitated by the negative control IgG and it remained in the unbound fraction.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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