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ZFIN ID: ZDB-FIG-110221-1
Noche et al., 2011 - Circadian rhythms in the pineal organ persist in zebrafish larvae that lack ventral brain. BMC Neuroscience   12:7 Full text @ BMC Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 1 avpl expression is absent in cyc mutants. Embryos were fixed at (A, B) 24 hours post-fertilization (hpf), (C-F) 46 hpf, and (G-I) 72 hpf and then processed for WISH with antisense probes for the indicated mRNAs. shh-expression in the ventral brain, including the hypothalamus (h, closed arrowhead) is (A) apparent in WT embryos but (B) absent in cyc mutants. (C, E, G, and I) avpl is expressed in the dorsal preoptic area (open arrowheads) and the ventral hypothalamus (closed arrowheads). (D, F, H, and J) Both avpl expression domains are eliminated in cyc mutants. (A-D, G, and H) are lateral views and (E, F, I, and J) are ventral views. The images in panels (C-J) are representative embryos of three independent experiments (n = 3 fish per experiment). avpl expression was also completely absent in cyc mutants at 47 hpf, Zeitgeber Time (ZT) 23.5; 52 hpf, ZT 5.5; 62 hpf, ZT 15.5, and 71.5 hpf, ZT 23.5. No circadian rhythm in avpl expression was detected in the WT larva processed in parallel (n≥4 larva per time point). Scale bars = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
avp WT standard conditions High-pec hypothalamus ISH
High-pec preoptic area ISH
Protruding-mouth hypothalamus ISH
Protruding-mouth preoptic area ISH
ndr2m294/m294 standard conditions High-pec Not Detected hypothalamus ISH
High-pec Not Detected preoptic area ISH
Protruding-mouth Not Detected hypothalamus ISH
Protruding-mouth Not Detected preoptic area ISH
shha WT standard conditions Prim-5 brain ventral region ISH
Prim-5 hypothalamus ISH
ndr2m294/m294 standard conditions Prim-5 Not Detected brain ventral region ISH
Prim-5 Not Detected hypothalamus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ndr2m294/m294 standard conditions Prim-5 whole organism lacks all parts of type brain ventral region, abnormal
High-pec whole organism lacks all parts of type brain ventral region, abnormal
Protruding-mouth whole organism lacks all parts of type brain ventral region, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Neurosci.