FIGURE

Fig. 1

ID
ZDB-FIG-110221-1
Publication
Noche et al., 2011 - Circadian rhythms in the pineal organ persist in zebrafish larvae that lack ventral brain
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

avpl expression is absent in cyc mutants. Embryos were fixed at (A, B) 24 hours post-fertilization (hpf), (C-F) 46 hpf, and (G-I) 72 hpf and then processed for WISH with antisense probes for the indicated mRNAs. shh-expression in the ventral brain, including the hypothalamus (h, closed arrowhead) is (A) apparent in WT embryos but (B) absent in cyc mutants. (C, E, G, and I) avpl is expressed in the dorsal preoptic area (open arrowheads) and the ventral hypothalamus (closed arrowheads). (D, F, H, and J) Both avpl expression domains are eliminated in cyc mutants. (A-D, G, and H) are lateral views and (E, F, I, and J) are ventral views. The images in panels (C-J) are representative embryos of three independent experiments (n = 3 fish per experiment). avpl expression was also completely absent in cyc mutants at 47 hpf, Zeitgeber Time (ZT) 23.5; 52 hpf, ZT 5.5; 62 hpf, ZT 15.5, and 71.5 hpf, ZT 23.5. No circadian rhythm in avpl expression was detected in the WT larva processed in parallel (n≥4 larva per time point). Scale bars = 50 μm.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Neurosci.