ZFIN Figure: Yin et al., 2010, Fig. 7

Fig. 7

Expression of mmp Genes and Their Inhibitors Is Altered in hand2 Mutants

(A) Comparison of mmp and timp expression in wild-type and han^s40-/- samples by quantitative real-time PCR (two independent experiments, three replicates of wild-type and han^s40-/- samples each time). The results are represented as relative expression levels that are normalized to β-actin (mean ± SEM).

(B) Proportion (mean ± SEM) of wild-type embryos (n = 29), wild-type embryos injected with 0.5 ng of mmp14a MO (n = 44), han^c99 heterozygotes (n = 55), and han^c99 heterozygotes injected with 0.5 ng of mmp14a MO (n = 70) showing gut-looping phenotypes according to foxa3 expression. Three independent experiments were conducted.

(A and B) Asterisks indicate statistical significance: *p < 0.05; **p < 0.01.

(C) Diagram of Hand2 regulating asymmetric LPM migration during zebrafish gut-looping morphogenesis. See also Figure S4.

Expression Data

Genes:	mmp14a timp2a timp2b
Fish:	pd24Tg hand2^s40/s40; pd24Tg
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hand2^c99/+ + MO4-mmp14a
Knockdown Reagent:	MO4-mmp14a
Observed In:	determination of intestine left/right asymmetry
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 18(6), Yin, C., Kikuchi, K., Hochgreb, T., Poss, K.D., and Stainier, D.Y., Hand2 Regulates Extracellular Matrix Remodeling Essential for Gut-Looping Morphogenesis in Zebrafish, 973-984, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell