FIGURE

Fig. 5

ID

ZDB-FIG-101124-6

Publication

Yin et al., 2010 - Hand2 Regulates Extracellular Matrix Remodeling Essential for Gut-Looping Morphogenesis in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Asymmetric LPM Migration Requires the Diminishment of Laminin by MMPs

(A and B) MMP inhibition blocked the asymmetric LPM migration. Tg(hand2:EGFP) embryos were treated with DMSO (A) or 10 μM GM6001 (B) from 24 hpf, and stained for GFP (green) and phalloidin (blue) at 30 hpf. Dashed lines outline the LPM.

(C and C′) Laminin (red) deposition was diminished near the Tg(hand2:EGFP)-expressing cells in the DMSO-treated controls (C, arrows), but persisted along the entire LPM/gut boundary in GM6001-treated embryos (C′, arrows).

(D) Proportions (mean ± SEM) of wild-type and han^c99+/- embryos showing gut-looping phenotypes according to foxa3 expression. Three independent experiments were conducted. Two hundred wild-type and 200 genotyped han^c99+/- embryos treated with DMSO or 5 μM GM6001 were examined. Asterisks indicate statistical significance: ^*p < 0.05; ^**p < 0.01.

(A–C′) Transverse sections, dorsal to the top. L, left; R, right. The scale bar represents 40 μm. See also Figure S2.

Expression Data

Antibody:	Ab1-lama1
Fish:	pd24Tg
Condition:	chemical treatment: pharmaceutical
Anatomical Term:	unspecified
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT hand2^c99/+
Condition:	chemical treatment: pharmaceutical
Observed In:	determination of intestine left/right asymmetry
Stage:	Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 18(6), Yin, C., Kikuchi, K., Hochgreb, T., Poss, K.D., and Stainier, D.Y., Hand2 Regulates Extracellular Matrix Remodeling Essential for Gut-Looping Morphogenesis in Zebrafish, 973-984, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell