FIGURE

Fig. S5

ID

ZDB-FIG-110111-10

Publication

Gjini et al., 2011 - Zebrafish Tie-2 shares a redundant role with Tie-1 in heart development and regulates vessel integrity

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Fig. S5

Zebrafish angiopoietin 2a binds to human TIE-2 receptor. (A) A comparison of zebrafish angiopoietin 1 and 2 and their orthologues in Fugu and Human. Clustal W alignment with the protein sequences of zebrafish (Danio rerio, Drer), Fugu-Fish (Takifugu rupripes, Trub) and of human (Homo sapiens, Hsap) shows that zebrafish and fugu-fish angiopoietin 1a and 1b are grouped together with human Angiopoietin 1. Zebrafish and fugu-fish angiopoietin 2b are clustered together with human Angiopoietin 2, whereas zebrafish and fugu-fish angiopoietin 2a is clustered as a separate group. (B) Zebrafish angiopoietin 2a (ang2a) binds to human Tie2-Fc. Full length zf ang2a cDNA was cloned in pCDNA3.1. ang2a, CA1 (Comp-Ang1) or empty pCDNA3.1 as a control were transiently expressed and 35S labelled in 293T cells. hTie2 protein (R&D) were used to pull-down 35S labeled angiopoietins from 293T conditioned medium. The bound proteins were separated on SDS-PAGE, and detected using autoradiography. The results show that hTie2-Fc binds to COMP-Ang1 as well as to Ang2a.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.