FIGURE

Fig. 5

ID

ZDB-FIG-110105-53

Publication

Oehlers et al., 2011 - A chemical enterocolitis model in zebrafish larvae that is dependent on microbiota and responsive to pharmacological agents

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Fig. 5

Zebrafish larvae mount a leukocytic response to trinitrobenzene sulfonic acid (TNBS) exposure. Left column is control and right column is TNBS-exposed. A: Comparison of neutrophil distribution in 6 days postfertilization (dpf) TG(mpx:EGFP)¹¹⁴. Larvae exposed to TNBS demonstrated a redistribution of leukocytes away from the CHT region. B: Comparison of confocal microscopy images of double transgenic TG(mpx:EGFP¹¹⁴, ifabp:RFP) larvae expressing EGFP in neutrophils and RFP in intestinal epithelial cells. C: Three-dimensional reconstruction of confocal microscopy data illustrating two angles. Scale bars in A and B represent 100 μm. CHT, caudal hematopoietic tissue. EGFP, enhanced green fluorescent protein. RFP, red fluorescent protein.

Expression Data

Genes:	EGFP GFP RFP
Fish:	i114Tg as200Tg; i114Tg
Condition:	chemical treatment: pharmaceutical
Anatomical Terms:	intestine epithelial cell neutrophil
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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