FIGURE

Fig. 5

ID

ZDB-FIG-101207-24

Publication

Teh et al., 2010 - Optogenetic in vivo cell manipulation in KillerRed-expressing zebrafish transgenics

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Fig. 5

Increased illumination increased apoptosis in the KR-expressing heart. (A-B) 8 min illumination of the 3dpf heart of SqKR15 embryo with intense green light reduced fluorescence intensity to 17%. (A) GFP and SqKR15 fluorescence in the ET33-mi3A:SqKR15 double transgenic embryo before and (B) after illumination. (C-D) same larvae one day after illumination: C - ET33-mi3A (control), D - SqKR15/ET33-mi3A (sample). TUNEL (+) cells in the heart of illuminated sample (D′) and control (C′), a day after illumination. (E-F) TUNEL staining of transverse sections of SqET33-mi3A larva (control) and (G-H) SqKR15/ET33-mi3A larva (sample), at different magnification one day after illumination. Examples of TUNEL (+) cells in the myocardial layer are boxed in white. All scale bars are 50 μm in length unless otherwise stated.

Expression Data

Genes:	EGFP KillerRed
Fish:	sqet33mi3AEt; sqKR15Et
Condition:	high light intensity, lighting conditions
Anatomical Term:	heart
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sqet33mi3AEt; sqKR15Et
Condition:	high light intensity, lighting conditions
Observed In:	atrial myocardium heart heart apoptotic process pericardium
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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