FIGURE

Fig. 4

ID

ZDB-FIG-101123-39

Publication

Kim et al., 2010 - PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts

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Fig. 4

PDGF signaling is sufficient to induce stress fibers and loss of cell-cell contacts in vitro. Sham-operated hearts were cultured in the presence of 0.5% serum for 3 d and then treated with 0.5% BSA as a control (n = 12 hearts) (A–C), PDGF-BB (n = 4 hearts) (D–F), PDGF-BB and PDGFR inhibitor (Inh.; 2 μM, n = 4 hearts) (G–I), PDGF-BB and ROCK inhibitor (2 μM, n = 4 hearts) (J–L), or PDGF-BB and PI3K inhibitor (2 μM, n = 4 hearts) (M–O) for 1 d (i.e., from day 3 to day 4 in culture) (actin, green; DAPI: blue; ZO1, red). Actin localized to the subcortical region is marked by arrowheads, and stress fibers are marked by arrows. Each individual explant was imaged in at least three different fields of view, and a representative image is shown. (Scale bar = 50 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA