FIGURE

Fig. 3

ID

ZDB-FIG-101122-46

Publication

Sarmah et al., 2010 - Inositol hexakisphosphate kinase-2 acts as an effector of the vertebrate Hedgehog pathway

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Fig. 3

IP6K2 is required for Hh target gene expression during development. (A–J) Whole-mount in situ hybridizations showing expression of Hh target genes in zebrafish embryos (24-hpf stage) injected with either control MO (column 1) or ip6k2^SPMO (column 2). Expression of shh (A–D) was slightly elevated, whereas ptc1 (E–H) and gli1 (I and J) expression was down-regulated in ip6k2^SPMO (B, D, F, H, and J) compared with controls (A, C, E, G, and I). (K–N) Whole-mount immunohistochemistry showing engrailed protein levels (detected by 4D9 antibody) in control MO (K) or ip6k2^SPMO (L) embryos at 24 hpf. Engrailed was not detected in ip6k2^SPMO embryos (L). M and N overlay engrailed immunohistochemistry and TO-PRO3-stained DNA. (O–R) Lateral views of embryos at 24 hpf stained for myosin heavy chain using F59 antibody to visualize slow muscle cells (O and P) and F-actin using rhodamine-phalloidin to reveal both fast and slow muscle cells (Q and R). Dorsal is toward the top and anterior to the left.

Expression Data

Genes:	gli1 ptch2 shha
Antibodies:	Ab-F59 Ab1-eng
Fish:	WT WT + MO3-ip6k2a
Knockdown Reagent:	MO3-ip6k2a
Anatomical Terms:	central nervous system ventral region floor plate horizontal myoseptum muscle pioneer notochord slow muscle cell somite
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-ip6k2a
Knockdown Reagent:	MO3-ip6k2a
Observed In:	slow muscle cell somite
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA