FIGURE

Fig. 3

ID

ZDB-FIG-101118-3

Publication

Chitramuthu et al., 2010 - Progranulin modulates zebrafish motoneuron development in vivo and rescues truncation defects associated with knockdown of Survival motor neuron 1

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Fig. 3

zfPGRN-A knockdown, rescue and over-expression. (A) Dose-dependent efficacy of morpholino (MO2) targeting the 5′ UTR of zfPGRN-A was assessed by Western blot analysis in comparison to its co-orthologue zfPGRN-B and actin. zfPGRN-A and B are represented by single bands of approximately 55 and 30 kDa, respectively. The Western blot analysis of MO knockdown efficacy is a representative of three independent injection sets. (B) Western blot analysis of protein extracts from embryos injected with 10 ng of zfPGRN-A MO1, 10 ng of zfPGRN-A MO2, zfPGRN-A-MO2 together with zfPGRN-A mRNA. Injection of 10 ng/embryo of the zfPGRN-A MO1 and MO2 effectively decreased zfPGRN-A translation, zfPGRN-A-MO together with mRNA resulted in normal zfPGRN-A translation. (C) Control experiments showing the results of the injection of 100pg hPGRN mRNA and 1ng gfp mRNA demonstrating translation into protein for both.

Expression Data

Genes:	grna grnb
Antibodies:	Ab1-grna Ab1-grnb
Fish:	WT WT + MO3-grna WT + MO4-grna
Knockdown Reagents:	MO3-grna MO4-grna
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Neurodegener.