Fig. 4

brca2^Q658X homozygotes are predisposed to testicular neoplasias, and brca2^Q658X and tp53^M214K mutations have collaborative effects on tumorigenesis. (A) Undifferentiated stromal cell tumor from a brca2^Q658X homozygote. (B) Undifferentiated stromal cell tumor from a brca2^Q658X homozygote with irregular bundles around poorly defined small tubular structures (Inset). (C) Papillary cystadenoma from a brca2^Q658X homozygote. (D) Seminoma from a brca2^Q658X homozygote. (E) Age at tumor onset is significantly lower in both brca2^Q658X homozygous;tp53^M214K homozygous zebrafish and brca2^Q658X heterozygous;tp53^M214K homozygous zebrafish compared with the tp53^M214K homozygous zebrafish parent line. (F) The ovary is a primary site for tumor development in brca2^Q658X heterozygous;tp53^M214K homozygous zebrafish, with oocyte entrapment (Inset). oc, oocyte; cr, cranial; d, dorsal; v, ventral; sb, swim bladder. (Scale bars: 100 μm in A and B; 200 μm in C and D; 1,000 μm in F; 50 μm in the Insets in A, B, C, and D; 100 μm in the Inset in F.)