FIGURE

Fig. 5

ID

ZDB-FIG-101111-5

Publication

Perälä et al., 2010 - Conservation, expression, and knockdown of zebrafish plxnb2a and plxnb2b

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Fig. 5

Normal development and locomotion of double plxnb2a and plxnb2b morphants. A: Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of the efficiency of the double knockdown using B2aMO and B2bMO morpholinos interfering with the splicing. Each morpholino was used at a concentration of 5 ng per embryo. E6* = 32 end of exon 6. B: Light microscopy images of lateral views of the double morphant as well as the controls at 5 days postfertilization (dpf). Ventral views of Alcian blue-stained 5 dpf double morphant and controls visualizing the cartilage structure. C-E: Results of the swimming performance assay from one representative replicate (zebrafish larvae per group n = 16-27). Graphs of the total distance moved (C), velocity (D), and angular velocity (E) are represented as mean and standard error of mean.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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