PUBLICATION
Conservation, expression, and knockdown of zebrafish plxnb2a and plxnb2b
- Authors
- Perälä, N., Peitsaro, N., Sundvik, M., Koivula, H., Sainio, K., Sariola, H., Panula, P., and Immonen, T.
- ID
- ZDB-PUB-100826-25
- Date
- 2010
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(10): 2722-2734 (Journal)
- Registered Authors
- Panula, Pertti, Peitsaro, Nina, Perälä, Nina, Sundvik, Maria
- Keywords
- plexins; neural development, zebrafish embryogenesis, Plexin B2 expression, Plexin B2 knockdown
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Axons/metabolism
- Behavior, Animal/physiology
- Cell Adhesion Molecules/chemistry
- Cell Adhesion Molecules/classification
- Cell Adhesion Molecules/genetics*
- Cell Adhesion Molecules/metabolism*
- Central Nervous System/embryology
- Central Nervous System/metabolism
- Cerebellum/embryology
- Cerebellum/metabolism
- Embryonic Development/genetics
- Embryonic Development/physiology
- Exons/genetics
- Gene Expression Regulation, Developmental*
- Introns/genetics
- Mesencephalon/embryology
- Mesencephalon/metabolism
- Molecular Sequence Data
- Nerve Tissue Proteins/chemistry
- Nerve Tissue Proteins/classification
- Nerve Tissue Proteins/genetics*
- Nerve Tissue Proteins/metabolism*
- Phylogeny
- Rhombencephalon/embryology
- Rhombencephalon/metabolism
- Zebrafish
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/classification
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 20737504 Full text @ Dev. Dyn.
Citation
Perälä, N., Peitsaro, N., Sundvik, M., Koivula, H., Sainio, K., Sariola, H., Panula, P., and Immonen, T. (2010) Conservation, expression, and knockdown of zebrafish plxnb2a and plxnb2b. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(10):2722-2734.
Abstract
In mice lacking Plexin B2, a receptor of the axon guidance molecules Semaphorin 4C and Semaphorin 4D, the closure of the neural tube and structural organization of the cerebellum are severely impaired. We cloned two Plexin B2 orthologs, plxnb2a and plxnb2b, in zebrafish, which is a widely used model for the development of the vertebrate central nervous system (CNS). The predicted proteins, Plexin B2a and Plexin B2b, contain all the conserved and functional domains of the plexin B-subfamily. During embryonic development, plxnb2a is expressed, e.g., in pharyngeal arches while plxnb2b expression is more confined to neuronal structures like the cerebellum. However, both plxnb2a and plxnb2b are expressed at the midbrain-hindbrain boundary, in the otic vesicles, facial ganglia, and pectoral fins. Knockdown of both plxnb2a and plxnb2b simultaneously (>95% and 45%, respectively) resulted in normal CNS structure, axon guidance and swimming performance of the morphants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping