FIGURE

Fig. 7

ID

ZDB-FIG-101111-39

Publication

Therapontos et al., 2010 - Zebrafish notch signalling pathway mutants exhibit trunk vessel patterning anomalies that are secondary to somite misregulation

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Fig. 7

Notch mutants exhibit ectopic filopodia on ISVs. A-H: Confocal microscope imaging of ISV outgrowth in 24 hpf fli1:EGFP (A) and aei (B), bea (C), and des (D) embryos, showing increased number of filopodia (black arrowheads) coming from the ISV in the mutants. Asterisk in C indicates filopodia arising from dorsal aorta. Scale bar = 25 μm. E: Mean ± s.e.m. filopodia numbers, counted over a 3-min period at 24 hpf, on ISVs for each Notch mutant analysed compared to controls (fli1:EGFP). (fli1:EGFP, n=4; aei, n=5;bea, n=7; des, n=5). P < 0.05 compared to fli1:EGFP (Student′s unpaired t-test). F,G: RT-PCR analysis of whole homogenized embryos, showing similar dll4 expression levels between WT and Notch mutant embryos at 24hpf. β-actin is a loading control. Two independent sets of RNAs are shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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