FIGURE

Fig. 5

ID

ZDB-FIG-101105-40

Publication

Jurynec et al., 2010 - SHIP2, a factor associated with diet-induced obesity and insulin sensitivity, attenuates FGF signaling in vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Maternal Ship2a attenuates FGF receptor-mediated pMAPK activation in the pre-gastrula embryo. (A-F) Anti-pMAPK immunoreactivity in oblong-sphere stage (3.7-4 hpf) WT, DMSO-treated control, maternal ship2a morphant and SU5402-treated embryos. (A,B) In WT and DMSO-treated control embryos, pMAPK is restricted to the dorsal side of the embryo. (C,D) Knockdown of maternal Ship2a causes a dramatic ventralward expansion of the pMAPK expression domain. (E,F) Inhibition of FGFR activity with 60 μM SU5402 completely inhibits pMAPK activation in both WT and maternal ship2a-morphant embryos. Confocal projections. Animal pole views with dorsal to the right.

Expression Data

Antibody:	Ab2-mapk
Fish:	AB AB + MO1-inppl1a AB + MO2-inppl1a
Condition:	chemical treatment: SU5402
Knockdown Reagents:	MO1-inppl1a MO2-inppl1a
Anatomical Terms:	blastodisc blastodisc dorsal region
Stage:	Oblong

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.