FIGURE

Fig. 3

ID

ZDB-FIG-100806-75

Publication

Francescatto et al., 2010 - The activation of membrane targeted CaMK-II in the zebrafish Kupffer's vesicle is required for left-right asymmetry

Other Figures

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Fig. 3

Left-right asymmetry is lost in CaMK-II morphants. (A) Lateral images of representative morphant embryos at 24 hpf and of Sox17-K⁴³A GFP CaMK-II cDNA-injected embryos at shield stage, 12 somites and 24 hpf include fluorescent (GFP) images. (B) Organ asymmetry was scored for embryos injected with 5 ng mismatch, 1.5 ng camk2aKAP, 1.5 ng camk2b2, 1.25 ng camk2g1 MOs and 150 pg Sox17-GFP-CaMK-II K⁴³A cDNA; n=87-233 per condition. (C) Representative dorsal views of camk2g1 morphant embryos stained with probes for cmlc2 (cardiac, 24 hpf), foxa3 (visceral organ, 48 hpf) and lefty1 (epithalamus, <24 somites). (D) Ca²⁺/CaM-dependent CaMK-II activity in pmoles/minute/mg were determined by peptide assays on 24 hpf lysates after injection with the indicated MO or cDNA.

Expression Data

Genes:	foxa3 lft1 myl7
Fish:	WT + MO1-camk2g1
Knockdown Reagent:	MO1-camk2g1
Anatomical Terms:	epithalamus gut heart tube liver pancreas
Stage Range:	20-25 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-camk2g1 WT + MO2-camk2a WT + MO2-camk2b2
Knockdown Reagents:	MO1-camk2g1 MO2-camk2a MO2-camk2b2
Observed In:	calcium/calmodulin-dependent protein kinase activity determination of digestive tract left/right asymmetry determination of left/right asymmetry in diencephalon determination of liver left/right asymmetry determination of pancreatic left/right asymmetry digestive system epithalamus heart jogging heart tube
Stage Range:	20-25 somites to Long-pec

Phenotype Detail

Acknowledgments

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