Fig. 2

Pujol-Martí et al., 2010 - Progressive neurogenesis defines lateralis somatotopy
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Simultaneous analysis of lateralis afferent neuron differentiation time and location in the posterior lateralis ganglion. A-C: Maximal projection of a Tg[HuC:Kaede] posterior lateralis ganglion at 60 hpf resulting from a BAPTI experiment conducted at 24 hpf. A: Merge of Tg[HuC:Kaede]green (B) and Tg[HuC:Kaede]red (C) signals. Early-differentiating neurons (converted at 24 hpf) are green and red (yellow), whereas late-differentiating neurons appear only green. D-G: Maximal projection of a posterior lateralis ganglion from a Tg[HuC:Kaede]red;HGn39D double transgenic fish at 60 hpf immunostained with the monoclonal antibody Zn-2. Confocal sections of a lateral (H-K) and a medial (L-O) plane of the same ganglion shown in D-G. P-S: Posterior lateralis ganglion from a Tg[HuC:Kaede]red;HGn39D double transgenic fish at 4 dpf immunostained with Zn-2. D,H,L,P: Merge of EGFP (E,I,M,Q), Tg[HuC:Kaede]red (F,J,N,R), and Zn-2 (blue) (G,K,O,S) fluorescent signals. The posterior lateralis ganglion was exposed to 405 nm light before image acquisition in order to convert all the Tg[HuC:Kaede]greento Tg[HuC:Kaede]red. This allowed us to distinguish the HGn39D(+) neurons (green) from the Tg[HuC:Kaede](+) neurons (red). White arrowheads indicate neuronal somata, which are only Tg[HuC:Kaede]red. Asterisk in P indicates the vagus ganglion, highlighted in Tg[HuC:Kaede] but not in HGn39D transgenic fish. All images are lateral views. Dorsal is up, anterior is left. Scale bars = 20 μm.

Expression Data
Anatomical Terms:
Stage Range: Pec-fin to Day 4

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.