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ZIRC
ZFIN ID: ZDB-FIG-100706-18
Chou et al., 2010 - Arterial and venous vessels are required for modulating developmental relocalization and laterality of the interrenal tissue in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   239(7):1995-2004 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 2 Effects of the ets1b antisense morpholino injection on the interrenal tissue and its neighboring vasculature. Confocal sections showing the interrenal tissue as detected by 3 β-Hsd activity staining (A-E), and the neighboring endothelium as labeled by green fluorescence (A′-E′), of 2-dpf Tg(fli1:EGFP)Y1 embryos uninjected (A), injected with ets1bMO (B-D), or with tnnt2MO (E), respectively. A″-E″: The merged images of 3 β-Hsd activity staining and GFP. A-E are dorsal views with anterior oriented to the top. F: The merged image of 3 β-Hsd staining and GFP of the same embryo as in E-E″, a lateral view with anterior to the left. B-B″, C-C″, D-D″: Classes I to III of interrenal phenotypes in the ets1b morphant, where the trunk endothelium was reduced and the axial vascular formation inhibited. Vascular ECs, but not the blood flow, are required for the convergence and laterality of the interrenal tissue. Yellow dotted lines indicate the position of the midline. Orange arrows, interrenal tissues; yellow arrowheads, angiogenic glomerulus; DA, dorsal aorta; PCV, posterior cardinal vein; ISV, intersegmental vessel. Scale bar = 50 μM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Long-pec dorsal aorta IFL
Long-pec posterior cardinal vein IFL
Long-pec pronephric glomerular capillary IFL
y1Tg + MO1-etv2 + MO2-etv2 standard conditions Long-pec vascular endothelium IFL
y1Tg + MO1-tnnt2a standard conditions Long-pec dorsal aorta IFL
Long-pec intersegmental vessel IFL
Long-pec posterior cardinal vein IFL
Long-pec pronephric glomerular capillary IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-etv2 + MO2-etv2 standard conditions Long-pec dorsal aorta absent, abnormal
Long-pec dorsal aorta decreased size, abnormal
Long-pec interrenal primordium bilateral, abnormal
Long-pec interrenal primordium left side unfused from interrenal primordium right side, abnormal
Long-pec posterior cardinal vein absent, abnormal
Long-pec vascular endothelium mislocalised, abnormal
y1Tg + MO1-tnnt2a standard conditions Long-pec adrenal gland development lacking processual parts cell migration, abnormal
Long-pec interrenal primordium position, normal
Long-pec pronephric glomerular capillary increased size, abnormal
Long-pec pronephric glomerulus right side unfused from pronephric glomerulus left side, abnormal
Long-pec vascular endothelium increased size, abnormal
Long-pec vascular endothelium mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.