FIGURE

Fig. S5

ID

ZDB-FIG-100628-56

Publication

Cerveny et al., 2010 - The zebrafish flotte lotte mutant reveals that the local retinal environment promotes the differentiation of proliferating precursors emerging from their stem cell niche

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Fig. S5

flo retinae exhibit increased proliferation in/near the CMZ. (A-H) Markers for the S (BrdU) and M (PH3) phases of the cell cycle are elevated in flo + p53MO embryos at 3 dpf. Frontal transverse cryosections stained with anti-BrdU (A,B, green) or anti-PH3 (C-H, green) to identify proliferating (A,B) or mitotic (C-H) cells. Nuclei in A,B are counterstained with To-Pro 633 (blue). (C-H) Of the sections examined (n=10 eyes, three representative examples of each are shown), WT retinae contained 2-8 PH3⁺ cells near the periphery, whereas the number of PH3⁺ cells in flo retinae ranged from 16-32. (I,J) Additionally, the region of the CMZ marked by cyclin D1 expression (ccnd1) is noticeably expanded when apoptosis is blocked in flo embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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