ZFIN ID: ZDB-FIG-100628-13
Reynolds et al., 2010 - VANGL1 rare variants associated with neural tube defects affect convergent extension in zebrafish. Mechanisms of Development   127(7-8):385-392 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 5-9 somites to 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 1 Activity of mouse Looptail VANGL1 mutant RNAs in zebrafish lacking tri. Panels A–E show lateral views of zebrafish at 2 days post fertilization. In Panel A, an uninjected wild-type fish is shown. In Panel B, a fish injected with tri-MO alone show a marked shortening of the trunk. In Panel C, VANGL1 RNA partially rescued the trunk phenotype induced by co-injection of tri-MO. Co-injection of VANGL1D259E (Panel D) or VANGL1S467N (Panel E) with tri-MO did not rescue the reduced body length phenotype induced by tri-MO.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 WT control 5-9 somites to 14-19 somites somite ISH
WT + MO1-vangl2 standard conditions 5-9 somites to 14-19 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-vangl2 standard conditions Long-pec trunk decreased length, abnormal
Long-pec whole organism anterior-posterior axis decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 127(7-8), Reynolds, A., McDearmid, J.R., Lachance, S., Marco, P.D., Merello, E., Capra, V., Gros, P., Drapeau, P., and Kibar, Z., VANGL1 rare variants associated with neural tube defects affect convergent extension in zebrafish, 385-392, Copyright (2010) with permission from Elsevier. Full text @ Mech. Dev.