FIGURE

Fig. 1

ID

ZDB-FIG-100621-5

Publication

Akhtar et al., 2009 - Use of Phospholipase A(2) for Antigen Retrieval in Zebrafish Whole-Mount Immunohistochemistry

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

PLA₂ retrieves cytoplasmic antigens. Patterns within the dissected intestine are shown with either a combination of PLA₂ and proteinase K or PLA₂ alone using 96 hpf embryos. Desmin IHC (A) reveals a robust and reproducible pattern with a combination of PLA₂ and a low concentration of proteinase K. Both longitudinal muscle (arrow) and circular (arrow head) are observed. Smooth muscle myosin heavy chain IHC (B) shows strong staining within circular muscle (arrow) with a combination of PLA₂ and proteinase K. Both beta-catenin (C) and cadherin (E) IHC display part or all of the pattern without antigen retrieval. Addition of an intermediate concentration of PLA₂ creates an even pattern of beta-catenin (D), while high levels of PLA₂ do not result in distortion of the cadherin pattern (F). In all panels, anterior is left and posterior is right. Scale bar is 20 μM at top left corner. PLA₂, phospholipase A₂; IHC, immunohistochemistry.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Zebrafish