FIGURE
            Fig. 2
- ID
 - ZDB-FIG-100621-6
 - Publication
 - Akhtar et al., 2009 - Use of Phospholipase A(2) for Antigen Retrieval in Zebrafish Whole-Mount Immunohistochemistry
 - Other Figures
 - All Figure Page
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                        Fig. 2
                    
                    
                
                
            
        
        
    
        
            
            
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 PLA2 retrieves nuclear antigens. Patterns within whole-mount embryos are revealed with PLA2 as the sole enzyme. The pattern of proliferation is identified in 54 hpf embryos injected with BrdU (A). Repeating patterns are observed at somite boundaries (arrowheads) and at somite midline (arrow). Phospho-Smad IHC demonstrates strong nuclear staining in the midbrain (arrowhead B) and hindbrain (arrow B). There is also strong staining in the developing ear (arrows in C and D). In all panels, anterior is left and posterior is right. Scale bar is 20 μM at top left corner.  | 
    
                
                    
                        Expression Data
                    
                    
                
                
            
        
        
    
        
            
            
            
            
    
    
                
                    
                        Expression Detail
                    
                    
                
                
            
        
        
    
        
            
                
            
        
    
    
    
                
                    
                        Antibody Labeling
                    
                    
                
                
            
        
        
    
        
            
                
            
        
    
    
    
                
                    
                        Phenotype Data
                    
                    
                
                
            
        
        
    
        
            
            
            
            
    
    
                
                    
                        Phenotype Detail
                    
                    
                
                
            
        
        
    
        
            
                
            
        
    
    
    
                
                    
                        Acknowledgments
                    
                    
                
                
            
        
        
    
        
            
            
                
                    
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