FIGURE

Fig. 7

ID

ZDB-FIG-100616-98

Publication

Insinna et al., 2010 - Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development

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Fig. 7

Dync1h1, Dync1i1, and Dnct1 immunoreactivity is associated with photoreceptor axonemes. Immunocytochemical localization of proteins in isolated adult zebrafish photoreceptor cells. (A) Immunocytochemical localization of Dynein1 heavy chain (Dync1h1) using antisera directed against three different epitopes. (B) Immunocytochemical localization of Dynein1 intermediate chain (Dync1i1), (C) Dynactin1/p150 (Dctn1), and (D) Synaptic vesicle protein 2 (SV2). (E) Immunostaining in which the primary antibody was omitted. In (A-C), cells were co-stained with acetylated α-tubulin (Tub) to highlight the axoneme. Color merged (Merge) and transmitted light images overlaid with Merge images (Trans) are shown for each panel. IS, inner segment; OS, outer segment. NP, no primary antibody. (F) Western blot analysis on detergent-extracted photoreceptor cytoskeleton (DEPC) fraction shows the specificity of the Dync1h1 antibody, as the Dync2 h2 antibody recognizes a discrete and faster migrating band.

Expression Data

Gene:	dync1h1
Antibodies:	Ab1-dync1h1 Ab1-tuba Ab2-dync1h1 Ab3-dync1h1
Fish:	WT
Anatomical Terms:	eye photoreceptor cell axoneme eye photoreceptor cell photoreceptor inner segment
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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