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ZFIN ID: ZDB-FIG-100616-24
Liu et al., 2010 - Expression of protocadherin-19 in the nervous system of the embryonic zebrafish. The International journal of developmental biology   54(5):905-911 Full text @ Int. J. Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5

Fig. 5 Pcdh19 expression in 18 and 24 hpf zebrafish embryos. (A-F) and (H-L) Whole mount embryos labeled with Pcdh19 cRNA. (G) is the head region of a whole mount embryo labeled with the anti-acytelated tubulin antibody. (A,E) Lateral views of the entire embryos (head towards the lower left corner). (B,F) Lateral views of the fore- and midbrains, while (C,H) are lateral views of the hindbrain (anterior to the left, dorsal up). (J,K,L) Dorsal views of the head region (anterior to the left). (D,I) Lateral views of the posterior half of the body and midtrunk region, respectively (anterior to the left). The arrowhead in (B,K), and the opposing arrows in (F,G) indicate the same region with reduced Pcdh19 expression, located between the posterior border of the optic tectum and boundary of the midhindbrains. The arrow in (B,C) point to the same area with reduced Pcdh19 expression, located immediately posterior to the cerebellum. The asterisk in (F,J,M) points to the same Pcdh19 expressing area in the dorsal thalamus. (M-P) Sections from whole mount embryos processed for Pcdh19 in situ hybridization. The apposing arrowheads in (F) indicate the plane of section for the image in (M) (anterior to the left), while (N-P) are cross sections (dorsal up) at the otic vesicle (ov), anterior-trunk, and mid-trunk regions, respectively. The opposing arrows in (N) indicate a band of tissue with reduced Pcdh19 staining. Other abbreviations: ac, anterior commissure; c, cerebellum; di, diencephalon; fp, floor plate of the spinal cord; h, hindbrain; hc, hair cells; hd, hypochord; le, lens; nc, notochord; or, optic recess; ot, optic tectum; p, pineal organ; poc, postoptic commissure; sc, spinal cord; te, telencephalon; teg, tegmentum; tpc, tract of posterior commissure; tpoc, tract of postoptic commissure. The remaining abbreviations are the same as in Fig. 4. Scale bars,100 μm for (A-M), and 50 μm for (N-P=.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pcdh19 WT control 14-19 somites diencephalon ISH
14-19 somites floor plate neural tube ISH
14-19 somites forebrain ISH
14-19 somites hindbrain ISH
14-19 somites hypochord ISH
14-19 somites midbrain ISH
14-19 somites spinal cord neural tube ISH
14-19 somites upper rhombic lip ISH
14-19 somites ventral telencephalon ISH
Prim-5 cerebellum ISH
Prim-5 dorsal thalamus ISH
Prim-5 floor plate spinal cord region ISH
Prim-5 hindbrain ISH
Prim-5 optic cup ISH
Prim-5 optic tectum ISH
Prim-5 otic vesicle ISH
Prim-5 solid lens vesicle ISH
Prim-5 spinal cord ISH
Prim-5 tegmentum ISH
Prim-5 telencephalon ISH
Prim-5 thalamus ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal The International journal of developmental biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Dev. Biol.