header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-100525-17
Hong et al., 2010 - Pre-gastrula expression of zebrafish extraembryonic genes. BMC Developmental Biology   10:42 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Shield

Fig. 5 Nodal-independent regulation of genes in the YSL. Whole mount in situ hybridizations on WT and MZoep embryos at the early-gastrula (6 hpf) stage. Lateral magnifications of the margin and YSL are shown throughout, except for lateral whole-embryo views in M′, N′, O′ and P′. No significant difference was seen in the YSL expression levels of mxtx1 in WT or MZoep embryos, as was previously reported (A-B). Similarly, no significant difference was seen between WT and MZoep embryos for the YSL expression of hnf4a (C-D), gpr137bb (E-F), or camsap1l1 (G-H). The expression of lhx1b (I-J) and sp5 (K-L) was substantially reduced in MZoep mutants. Similarly, concentrated expression of zgc:110712 near the margin was absent in MZoep embryos (M, M′, N, N′). The gata6 gene is expressed both in the YSL and margin (O and O′) and the margin expression domain is eliminated in MZoep mutants, whereas the YSL expression domain persists (P and P′).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
camsap2a AB standard conditions Shield yolk syncytial layer ISH
tdgf1tz257/tz257 standard conditions Shield yolk syncytial layer ISH
gata6 AB standard conditions Shield margin ISH
Shield yolk syncytial layer ISH
tdgf1tz257/tz257 standard conditions Shield Not Detected margin ISH
Shield yolk syncytial layer ISH
gpr137 AB standard conditions Shield yolk syncytial layer ISH
tdgf1tz257/tz257 standard conditions Shield yolk syncytial layer ISH
hnf4a AB standard conditions Shield yolk syncytial layer ISH
tdgf1tz257/tz257 standard conditions Shield yolk syncytial layer ISH
lhx1b AB standard conditions Shield margin ISH
tdgf1tz257/tz257 standard conditions Shield margin ISH
mxtx1 AB standard conditions Shield yolk syncytial layer ISH
tdgf1tz257/tz257 standard conditions Shield yolk syncytial layer ISH
sp5a AB standard conditions Shield margin ISH
tdgf1tz257/tz257 standard conditions Shield margin ISH
krt99 AB standard conditions Shield margin ISH
tdgf1tz257/tz257 standard conditions Shield Not Detected margin ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.