Fig. S1
The centriole templates early ciliary axoneme assembly. High magnifications views of cross sections through the hindbrain region of embryos at the neural keel stage, immunolabeled with (A?A″) anti-Ace-tub (ciliary axoneme, in green) and anti-Glu-tub (cytoplasmic MT and ciliary axoneme, in red) or with (B?B″) anti-γ-tub (centrosome, in green) and anti-Glu-tub. Nuclei are labeled with DAPI, in blue. (C) Illustration of cellular organelles visualized using different antibodies. Symbols: arrowheads indicate axonemes; arrows show the centrosome. Scale bar: 5 μm. |
Reprinted from Developmental Biology, 341(2), Hong, E., Jayachandran, P., and Brewster, R., The polarity protein Pard3 is required for centrosome positioning during neurulation, 335-345, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.