Fig. 4
Pard3 is required for apical localization of the centrosome. Sections of control (A?A″, C?C″, E?E″) and pard3 MO-injected (B?B″, D?D″, F?F″) embryos at the early rod (6?7 som, A?B″), late rod (12?13 som, C?D″) and neural tube (24 hpf, E?F″) stages immunolabeled with anti-Glu and anti-γ-tub. Ovals and rectangles in (A?F): areas magnified in panels A′?F′ and A″?F″ respectively; white lines: midline; bracketed lines: MTs extending towards the apical cortex; arrowheads: centrosomes. Scale bar: 10 μm. |
Fish: | |
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Knockdown Reagent: | |
Observed In: | |
Stage Range: | 5-9 somites to Prim-5 |
Reprinted from Developmental Biology, 341(2), Hong, E., Jayachandran, P., and Brewster, R., The polarity protein Pard3 is required for centrosome positioning during neurulation, 335-345, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.