FIGURE

Fig. 8

ID
ZDB-FIG-100520-18
Publication
Branam et al., 2010 - Zebrafish Chordin-like and Chordin Are Functionally Redundant in Regulating Patterning of the Dorsoventral Axis
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Fig. 8

Synergistic effects of Chl- and Chd-MOs in inducing ventralization does not involve p53-mediated off-target effects. (A) A comparison is shown of 24-hpf embryos treated with 0.38 mM StCon-MO, 0.1 mM Chl 5′1-MO, 0.026 mM Chd-MO plus 0.25 mM p53-MO, 0.1 mM Chl 5′1-MO plus 0.026 mM Chd-MO, or with 0.1 mM Chl 5′1 plus 0.026 mM Chd-MO and 0.25 mM p53-MO. (B) Quantification of phenotypes at 24 hpf. Severity of dorsalization (D) is numerically classified as per Mullins et al., 1996. Degree of ventralization (VE) was not numerically classified. (C–D) Whole-mount in situ hybridization is shown for expression of the dorsal marker Chl at 30% epiboly for embryos treated with 0.38 mM StCon-MO (C), 0.1 mM Chl 5′1-MO plus 0.25 mM p53-MO (D), 0.026 mM Chd-MO plus 0.25 mM p53-MO (E), or 0.1 mM Chl 5′1 plus 0.026 mM Chd-MO and 0.25 mM p53-MO.

Expression Data
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 30%-epiboly

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 30%-epiboly to Prim-5

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 341(2), Branam, A.M., Hoffman, G.G., Pelegri, F., and Greenspan, D.S., Zebrafish Chordin-like and Chordin Are Functionally Redundant in Regulating Patterning of the Dorsoventral Axis, 444-458, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.