Figure 4. ApoE expression is enhanced during regeneration of the pectoral fin. ApoE expression was determined A:18 hr; B: 24 hr; C: 3 days; D: 4 days; E: 6 days; F: 14 days after amputation. The plane of amputation is indicated by the thick red arrow. A-E: apoE is strongly expressed in the wound epidermis at the apex of each fin ray during the first 4 to 6 days of regeneration. F: 14 days after amputation, apoE expression has decreased to levels comparable to control non-amputated pectoral fin. A thin red arrow indicates a differentiating lepidotrichia in D,E and a newly formed bifurcation in F. ae, apical epidermis; b, blastema; we, wound epidermis. Scale bar = 200 m.
|