Fig. S5

Effect of Fgf-soaked bead transplantation on the her1 expression pattern. (A) Comparison of her1 expression pattern between control and Fgf bead transplanted sides. Each side of the same sample is shown. The anterior her1 stripe was anteriorly shifted and the posterior her1 expression domain became broadened (n=7/9). The light-blue and magenta dots represent the axial levels of the anterior stripe on control and bead transplanted sides, respectively. White arrows indicate the width of the posterior expression of her1 on each side. The transplantation was performed under the same conditions as in Fig. 2C-G except for the stage of transplantation (∼7.0 hpf). (Ba-c) Simulation of an increased frequency gradient. (a,b) The intrinsic (a) and observed (b) frequency. The intrinsic frequency for the Fgf bead transplantation case was fixed at 0.6 in the cells i=40-50 (red). (c) The simulated stripe pattern of the control (left) and bead transplanted (right) cases, when the stripe first appeared under each condition. The simulated spatial pattern recapitulates well the outcome of Fgf bead transplantation. The anterior stripe was anteriorly shifted and the posterior stripe became broader. The light-blue and magenta dots represent the position of the anterior stripe in the control and bead transplanted cases, respectively. White arrows indicate the width of the posterior stripes. The simulation is based on the same equation as in Fig. 3C. For the control simulation, the anterior non-oscillatory cells were not coupled with the posterior oscillatory cells. t under the panels indicates the elapsed time.