FIGURE

Fig. S6

ID

ZDB-FIG-100504-71

Publication

Mao et al., 2009 - Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb

Other Figures

All Figure Page

Back to All Figure Page

Fig. S6

erm;pea3;etv5 Morpholino Knockdown Results in Expansion of Shh Expression within the Fin Bud
(A) Wild-type series of Shh expression in the zebrafish embryo, from 29 hours post fertilization (hpf) to 48 hpf. A higher magnification image of the fin is shown below; scale bar represents 0.02mm.
(B) Embryos were injected with triple MO mix or mismatch MO mix at the 1-2 cell stage and Shh expression assessed 48 hours post-fertilization. Shh expression in mismatch MO control embryos (n=40) appeared identical to wild-type embryos (n=20). In 20/52 experimental MO injected embryos (pea3;erm;etv5 MO_a), an increase in the intensity and domain of Shh expression was observed in the distal fin bud. For embryos where fin bud development was significantly delayed (pea3;erm;etv5 MO_b; n=20/52), an expanded domain of Shh was still observed relative to age-matched controls, the ectopic anterior Shh expression domain is arrowed. No change in Shh expression was observed in 9/52 embryos, while a decrease/loss of Shh was recorded in 3/52 embryos that also exhibited severe developmental defects.

Expression Data

Gene:	shha
Fish:	WT WT + MO1-etv5a + MO2-etv4 + MO2-etv5b
Knockdown Reagents:	MO1-etv5a MO2-etv4 MO2-etv5b
Anatomical Term:	pectoral fin bud
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 16(4), Mao, J., McGlinn, E., Huang, P., Tabin, C.J., and McMahon, A.P., Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb, 600-606, Copyright (2009) with permission from Elsevier. Full text @ Dev. Cell