ZFIN Image: Mao et al., 2009, Fig. S6

Image

Figure Caption

Fig. S6 erm;pea3;etv5 Morpholino Knockdown Results in Expansion of Shh Expression within the Fin Bud
(A) Wild-type series of Shh expression in the zebrafish embryo, from 29 hours post fertilization (hpf) to 48 hpf. A higher magnification image of the fin is shown below; scale bar represents 0.02mm.
(B) Embryos were injected with triple MO mix or mismatch MO mix at the 1-2 cell stage and Shh expression assessed 48 hours post-fertilization. Shh expression in mismatch MO control embryos (n=40) appeared identical to wild-type embryos (n=20). In 20/52 experimental MO injected embryos (pea3;erm;etv5 MO_a), an increase in the intensity and domain of Shh expression was observed in the distal fin bud. For embryos where fin bud development was significantly delayed (pea3;erm;etv5 MO_b; n=20/52), an expanded domain of Shh was still observed relative to age-matched controls, the ectopic anterior Shh expression domain is arrowed. No change in Shh expression was observed in 9/52 embryos, while a decrease/loss of Shh was recorded in 3/52 embryos that also exhibited severe developmental defects.

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Cell, 16(4), Mao, J., McGlinn, E., Huang, P., Tabin, C.J., and McMahon, A.P., Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb, 600-606, Copyright (2009) with permission from Elsevier. Full text @ Dev. Cell