Fig. 8

Competition experiment for module D probes 4 and 5. I. EMSA competition experiment of module D-probe 4. The gel image was obtained with probe only (lane 1) and with 5 and 15 μg nuclear extract (lane 2, 3) to detect the binding protein. The sequences (wild-type or Pax6.1 binding site mutant) for competition are shown below. Three different amounts (10x, 20x and 50x) of competitor were added to the binding reaction with 15 μg nuclear extract. Lanes 4, 5 and 6 are competition experiments with wild-type competitors at 10x, 20x and 50x, respectively, and lanes 7, 8 and 9 are competition experiments with the Pax6.1 site mutant at 10x, 20x and 50x, respectively. II. EMSA competition experiment of module D-probe 5. The gel image was obtained with probe only (lane 1) and with 5 and 15 μg nuclear extract (lane 2 and 3) to detect the binding protein. The sequences (wild-type or FOX binding site mutant) for competition are shown below. Three different amounts (10x, 20x and 50x) of competitor were added to the binding reaction with 15 μg nuclear extract. Lanes 4, 5 and 6 are competition experiments with wild-type competitors at 10x, 20x and 50x, respectively, and lanes 7, 8 and 9 are competition experiments with the FOX site mutant at 10x, 20x and 50x, respectively.