Fig. 5

Constructs and representative images for module D. I. Different constructs used to study the function of six3a module D. The names of those constructs were given by the length of the DNA fragment left after 5′ or 3′ deletion. The number after D indicates the length of module D left after 5′ deletion. The small triangle indicates the length of module D left after 3′ deletion. Followed by each construct are the summary of the GFP expression level at different times (8, 11 and 24 hpf) and regions (eyes, forebrain and ectopic) as plus sign (+) to indicate different levels of GFP. The minus sign (-) indicates the absence of GFP expression. II. The percentages of embryos expressing GFP from different batches are shown with the total numbers of embryos, and three time points: 8 hpf (orange), 11 hpf (blue) and 24 hpf (red). III. Representative GFP expression patterns for each construct in lateral views at 24 hpf (A-K) or 8 hpf (L-O) with the anterior position marked with an arrow. (A) D184-GFP. (B) D141-Bp-GFP. (C) D105-Bp-GFP. (D) D69-Bp-GFP. (E) D30-Bp-GFP. (F) Dorsal view of D28(2)-Bp-GFP. (G) D184(triangle)74-Bp-GFP. (H) D184(triangle)30-Bp-GFP. (I) D184(triangle)42-Bp. (J) D105(triangle)42-Bp-GFP. (K) D105(triangle)30-Bp-GFP. (L) D105, (M) D184, (N) D184(triangle)42 and (O) D69(triangle)30. The last panel shows the sequence in module D. Conserved sequences in module D were aligned among zebrafish, Tetraodon, fugu and medaka, and the primers for making the deletion constructs are shown above the sequences. See legend in Fig 4 for others symbols.