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ZIRC
ZFIN ID: ZDB-FIG-100211-39
Rikin et al., 2010 - The tbx/bHLH transcription factor mga regulates gata4 and organogenesis. Developmental dynamics : an official publication of the American Association of Anatomists   239(2):535-547 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 8 Knockdown of gata4 can rescue partially the mga morphant heart tube phenotype. A-C: Shown are representative embryos that are wildtype (A), injected with only the mga-specific MO1 (B), or injected first with MO1 and subsequently with the gata4 morpholino (C). The ratios shown represent the number of embryos with an apparently normal (non-edemic) phenotype in a representative experiment (which was reproduced several times). Note that the cardiac edema present in 100% of the single morphants (B) is rescued in a significant percentage (35%) of the co-injected embryos. The brain defect is also largely rescued, although in contrast, the regression in the yolk stalk extension persists. D-L: Similar experiments were carried out and imaged using embryos derived from cmlc2:gfp transgenic fish. In these experiments, initial heart looping was rescued in double-knockdown embryos compared to the mga morphants as shown in F (51/128, 40%) or I (32/77, 42%) at 3 dpf. J-L: Although it is not normal, a similar rescue (35/105, 33%) of heart looping was scored at 5 dpf. All views are lateral, dorsal to the top, and anterior to the right except for G-I, which are ventral, anterior to the right. HT, heart; YSE, yolk stalk extension.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg standard conditions Protruding-mouth heart IFL
Day 5 heart IFL
twu34Tg + MO1-gata4 + MO1-mgaa standard conditions Protruding-mouth heart IFL
Day 5 heart IFL
twu34Tg + MO1-mgaa standard conditions Protruding-mouth heart IFL
Day 5 heart IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
twu34Tg + MO1-gata4 + MO1-mgaa standard conditions Protruding-mouth extension degenerate, abnormal
Protruding-mouth pericardium composition, normal
Day 5 extension degenerate, abnormal
Day 5 pericardium composition, normal
twu34Tg + MO1-mgaa standard conditions Protruding-mouth extension degenerate, abnormal
Protruding-mouth heart looping disrupted, abnormal
Protruding-mouth pericardium edematous, abnormal
Day 5 extension degenerate, abnormal
Day 5 heart looping disrupted, abnormal
Day 5 pericardium edematous, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.