FIGURE

Fig. 2

ID

ZDB-FIG-091217-83

Publication

den Broeder et al., 2009 - Generation and characterization of FMR1 knockout zebrafish

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Fig. 2

Phenotypic assays on wild-type and fmr1 mutant embryos.

A) Wild type and mutant embryos were analyzed using whole mount in situ hybridisation using probes against dlx-2a, axial and islet-1. B) The width of Meckel′s cartilage was measured in wild type (n = 9) and MZ fmr1 mutant (n = 11) embryos. The angle of this structure with regard to the anterior-posterior axis was also measured in wild-type (n = 6) and fmr1 mutant (n = 9) embryos. Indicated errors represent SD. C) Neurite branching was measured on Rohon-Beard neurites using the monoclonal antibody zn-12. Plotted is the branching frequency per 1000 μm in both wild-type and MZ stop mutant embryos. In total n = 25 neurites (wild-type) and n = 28 neurites (MZfmr1) were traced in a total of 8 embryos of each genotype. Error bars represent SD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fmr1^{hu2787/hu2787} (TL)
Observed In:	Meckel's cartilage Rohon-Beard neuron neuron projection
Stage:	Day 5

Phenotype Detail

Acknowledgments

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