ZFIN ID: ZDB-FIG-091217-82
den Broeder et al., 2009 - Generation and characterization of FMR1 knockout zebrafish. PLoS One   4(11):e7910 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 1 Fmr1 mutant alleles.

A) Illustration of the fmr1 gene product. The different domains are indicated, along with the sites where isolated mutant alleles will affect the protein. B) Whole mount in situ hybridisation with an fmr1 specific probe. C) Immuno staining of wild type and mutant brain sections using Fmr specific antibodies. Some Purkinje cells in the mutant have been outlined. D) Brain lysates were analyzed by western blot, using an Fmr specific antibody. Lanes 1 and 3 contain wild type samples. Lane 2 contains hu2787/hu2787 lysate. Lane 4 contains hu2787/hu2898 lysate. The upper arrow points at Fmr. The lower arrow points at an a-specific band that serves as a loading control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fmr1 Ab1-fmr1 WT standard conditions Adult brain WB
Ab1-fmr1 Adult Purkinje cell IHC
Ab1-fmr1 Adult telencephalon neuron IHC
Ab1-fmr1 fmr1hu2787/+; fmr1hu2898/+(TL) standard conditions Adult Not Detected brain WB
Ab1-fmr1 Adult Not Detected Purkinje cell IHC
Ab1-fmr1 Adult Not Detected telencephalon neuron IHC
Ab1-fmr1 fmr1hu2787/hu2787(TL) standard conditions Adult Not Detected brain WB
Ab1-fmr1 Adult Not Detected Purkinje cell IHC
Ab1-fmr1 Adult Not Detected telencephalon neuron IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One