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ZFIN ID: ZDB-FIG-091214-74
Felip et al., 2009 - Evidence for two distinct KiSS genes in non-placental vertebrates that encode kisspeptins with different gonadotropin-releasing activities in fish and mammals. Molecular and Cellular Endocrinology   312(1-2):61-71 Full text @ Mol. Cell. Endocrinol.
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 4 RT-PCR analysis of medaka and zebrafish KiSS-1 and KiSS-2 expression in tissues from male and female adult fish. Total RNA extracted from 3 to 6 pooled tissues was reverse-transcribed and amplified by PCR. mdef1α and zfef1α were used as an internal control to verify the integrity of the RNAs. (C-) Negative control for PCR which were performed with sterile water as template.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
kiss1 WT standard conditions Adult eye RTPCR
Adult female organism brain RTPCR
Adult gill RTPCR
Adult integument RTPCR
Adult intestine RTPCR
Adult male organism brain RTPCR
Adult ovary RTPCR
Adult spleen RTPCR
Adult testis RTPCR
kiss2 WT standard conditions Adult female organism brain RTPCR
Adult male organism brain RTPCR
Adult ovary RTPCR
Adult testis RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Molecular and Cellular Endocrinology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and Cellular Endocrinology, 312(1-2), Felip, A., Zanuy, S., Pineda, R., Pinilla, L., Carrillo, M., Tena-Sempere, M., and Gómez, A., Evidence for two distinct KiSS genes in non-placental vertebrates that encode kisspeptins with different gonadotropin-releasing activities in fish and mammals, 61-71, Copyright (2009) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.