FIGURE

Fig. 3

ID

ZDB-FIG-091130-2

Publication

Hippe et al., 2009 - The interaction of nucleoside diphosphate kinase B with Gbetagamma dimers controls heterotrimeric G protein function

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Fig. 3

Gβ₁ and Gβ_1like depletion in zebrafish embryos. Zebrafish embryos were injected with MO-Ctrl (4 ng), MO-Gβ₁ (3 ng) and MO-Gβ_1like (3 ng) singly or in combination (3 + 3 ng). (A) Immunoblot analysis of lysates prepared 72 hpf using specific antibodies against NDPK B, Gβ₁ and Gγ₂. β-actin served as loading and specificity control. (B) Relative NDPK activity (normalized to MO-Ctrl) was quantified as formation of ³H-GTP from ³H-GDP and ATP in lysates of embryos injected as indicated. Data are means ± SEM, n = 3, *, P < 0.05 vs. MO-Ctrl.

Expression Data

Genes:	gnb1a gng2 nme2b.1
Antibody:	Ab1-nme2
Fish:	WT WT + MO1-gnb1a WT + MO1-gnb1b WT + MO1-gnb1a + MO1-gnb1b
Knockdown Reagents:	MO1-gnb1a MO1-gnb1b
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gnb1a + MO1-gnb1b
Knockdown Reagents:	MO1-gnb1a MO1-gnb1b
Observed In:	GTP biosynthetic process
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA