Alterations in morphology and gene expression of pipetail (pptta98, A-J,L) and kugelig (kggtl240, C,K) mutant embryos, compared to wild type (+). (A) 10-somite stage, lateral view. (B) 10- somite stage, dorsal view: in ppt mutants, the notochord is undulated and the somites are condensed along the anteroposterior axis. Somites 9 and 10 and the presomitic mesoderm show a slight lateral expansion. (C) 24-somite stage, lateral view. (D) Day 5, ventral view of head: the head skeleton forms but fails to extend beyond the eyes, leading to a slight broadening of the head in ppt mutants. (E) Day 5, lateral view of tip of tail: the notochord and the neurocoel are slightly kinked at the end of the tail; the ventral gap of the melanophore stripe is present. (F-K) Dorsal view on spread embryo, anterior left: (F) wnt5, 5-somite stage: the expression of wnt5 is significantly reduced in somites and tailbud of ppt mutants. (G) wnt3, 5-somite stage. (H) ntl, 8-somite stage. (J) myoD, 12-somite stage: all somites are condensed, the posterior somites are laterally expanded in ppt mutants. (K) myoD, 16-somite stage: the posterior somites are condensed but not laterally expanded in kgg mutants. (L) eve1, 28- somite stage, lateral view on tail.
|
