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Functional interaction between Ier2 and Fibp1. (A–D) Lateral views at 24 hpf. The ier2 MO-injected embryo has a short trunk (B) compared with the control (A). The ier2 MO phenotype was rescued by coinjection with zebrafish ier2 mRNA (C) and human ier2 mRNA (D). (E–S). Lateral views at 48 hpf. (E–L) Knock down phenotypes. Low doses of ier2 MO (F) or fibp1 MO (H) have no phenotype, whereas effective doses of ier2 MO (G) and fibp1 MO (I) show very similar phenotypes. Coinjection with both MOs at low dose mimics the high-dose phenotype (J). ier2 MO is rescued by fibp1 RNA (K), and fibp1 MO is rescued by ier2 RNA (L). (M–S) Ier2 and fibp1 overexpression phenotypes. (M) Control RNA-injected embryos. (N and O) ier2 RNA injection phenotypes at low dose and effective dose. (P and Q) fibp1 RNA injection phenotypes at low dose and effective dose. (R) Coinjection of low dose of ier2 plus fibp1 RNA. (S) Injection of human hier2 RNA.
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