|ZFIN ID: ZDB-PUB-090204-7|
FGF-dependent left-right asymmetry patterning in zebrafish is mediated by Ier2 and Fibp1
Hong, S.K., and Dawid, I.B.
|Source:||Proceedings of the National Academy of Sciences of the United States of America 106(7): 2230-2235 (Journal)|
|Registered Authors:||Dawid, Igor B., Hong, Sung-Kook|
|Keywords:||ciliogenesis, Kupffer's vesicle, laterality|
|PubMed:||19164561 Full text @ Proc. Natl. Acad. Sci. USA|
Hong, S.K., and Dawid, I.B. (2009) FGF-dependent left-right asymmetry patterning in zebrafish is mediated by Ier2 and Fibp1. Proceedings of the National Academy of Sciences of the United States of America. 106(7):2230-2235.
ABSTRACTEstablishment of left-right asymmetry in vertebrates requires nodal, Wnt-PCP and FGF signaling and involves ciliogenesis in a laterality organ. Effector genes through which FGF signaling affects laterality have not been described. We isolated the zebrafish ier2 and fibp1 genes as FGF target genes and show that their protein products interact. Knock down of these factors interferes with establishment of organ laterality and causes defective cilia formation in Kupffer's Vesicle, the zebrafish laterality organ. Cilia are also lost after suppression of FGF8, but can be rescued by injection of ier2 and fibp1 mRNA. We conclude that Ier2 and Fibp1 mediate FGF signaling in ciliogenesis in Kupffer's Vesicle and in the establishment of laterality in the zebrafish embryo.